CrkL 抗体
Quick Overview for CrkL 抗体 (ABIN7469155)
抗原
See all CrkL (CRKL) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- IP/MS validation was supported by references (PMID:30377387)
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交叉反应
- 人, 小鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human CrkL. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: mouse brain , 293T , A431 , HeLa , HepG2 Validation: IP/MS, Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.33 mg/mL
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缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
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别名
- CRK like proto-oncogene, adaptor protein
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背景
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Synonyms: CRK like proto-oncogene, adaptor protein
Background: This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
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分子量
- 34 kDa
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基因ID
- 1399
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UniProt
- P46109
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途径
- Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor
抗原
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