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CrkL 抗体 (C-Term)

Verified CRKL 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN184660
发货至: 中国
  • 抗原 See all CrkL (CRKL) 抗体
    CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
    抗原表位
    • 22
    • 19
    • 15
    • 14
    • 12
    • 8
    • 8
    • 8
    • 7
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    适用
    • 96
    • 70
    • 26
    • 5
    • 1
    • 1
    人, 小鼠
    宿主
    • 116
    • 8
    • 1
    • 1
    山羊
    克隆类型
    • 113
    • 12
    多克隆
    标记
    • 44
    • 11
    • 10
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This CrkL antibody is un-conjugated
    应用范围
    • 76
    • 44
    • 26
    • 26
    • 16
    • 14
    • 12
    • 12
    • 6
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    CRKL
    序列
    KIFDPQNPDE NE
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    免疫原
    Peptide with sequence C-KIFDPQNPDENE, from the C Terminus of the protein sequence according to NP_005198.1.
    亚型
    IgG
    Top Product
    Discover our top product CRKL Primary Antibody
  • 应用备注

    Immunohistochemistry: In paraffin embedded Human Thymus shows staining of cellular membranes in thymocytes. Recommended concentration: 2.5-3.8 μg/mL.

    Western Blot: Approx 37 kDa band observed in lysates of cell lines K562, Daudi and NIH3T3 (calculated MW of 33.8 kDa according to Human NP_005198.1 and Mouse NP_031790.2 ). Recommended concentration: 0.1-1 μg/mL. Primary incubation 1 hour at room temperat

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.<

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
    别名
    CRKL (CRKL 产品)
    别名
    CRKL antibody, wu:fa55g05 antibody, wu:fj60e01 antibody, zgc:63469 antibody, 1110025F07Rik antibody, AA589403 antibody, AI325100 antibody, Crkol antibody, CRK like proto-oncogene, adaptor protein antibody, v-crk avian sarcoma virus CT10 oncogene homolog-like antibody, CRKL antibody, crkl antibody, Crkl antibody
    背景
    CRKL, v-crk sarcoma virus CT10 oncogene homolog (avian)-like, v-crk avian sarcoma virus CT10 oncogene homolog-like, OTTHUMP00000198539
    基因ID
    1399
    NCBI登录号
    NP_005198
    途径
    Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor
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