NDC80 抗体 (pSer55)
Quick Overview for NDC80 抗体 (pSer55) (ABIN7468645)
抗原
See all NDC80 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- pSer55
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交叉反应
- 人, Kangaroo rat (Dipodomys sp.), 小鼠, 大鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide surrounding phospho Ser55 of human Hec1. The exact sequence is proprietary.
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亚型
- IgG
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anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 545-642) antibody
NDC80 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1A10 unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (C-Term) antibodyNDC80 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (C-Term) antibodyVerified NDC80 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) antibodyNDC80 适用: 人 WB, ICC, DB 宿主: 小鼠 Monoclonal 2497C3a unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 508-539) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal RB55345 unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 594-628) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal RB53951 unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 1-642) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 1-642) antibodyNDC80 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) (AA 343-642) antibodyNDC80 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NDC80 Kinetochore Complex Component Homolog (NDC80) antibodyNDC80 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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应用备注
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Recommended Starting Dilutions:
For WB: 1:500-1:3000
For IP: Use at a dilution of 2-4 μg/mL for 1 mg cell extract
For ICC/IF: Use at a dilution of 1:300
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher. -
说明
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Positive Control: HeLa , Blocking peptide (GTX70017-PEP) , HeLa (100 ng/ml Nocodazole treatment for 24 hr) Validation: KO/KD, Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.26 mg/mL
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缓冲液
- 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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别名
- NDC80 kinetochore complex component
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背景
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Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Background: HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM]
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分子量
- 80 kDa
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基因ID
- 10403
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UniProt
- O14777
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途径
- Maintenance of Protein Location
抗原
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