NDC80 抗体 (pSer76, pSer77)
Quick Overview for NDC80 抗体 (pSer76, pSer77) (ABIN7468643)
抗原
See all NDC80 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer76, pSer77
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交叉反应
- 人
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纯化方法
- Affinity purified using antigen
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免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Hec1 (phospho Ser 76/77). The exact sequence is proprietary.
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亚型
- IgG
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应用备注
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Recommended Starting Dilutions:
For WB: Use at a dilution of 1:300
For ICC/IF: Use at a dilution of 1:150
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher. -
说明
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Positive Control: HeLa , Blocking peptide (GTX70015-PEP)
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.079 mg/mL
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缓冲液
- 1X PBS, No Preservative
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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别名
- NDC80 kinetochore complex component
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背景
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Synonyms: NDC80 kinetochore complex component , HEC , HEC1 , HsHec1 , KNTC2 , TID3 , hsNDC80
Background: This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
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分子量
- 74 kDa
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基因ID
- 10403
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UniProt
- O14777
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途径
- Maintenance of Protein Location
抗原
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