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HMGB1 抗体 (N-Term)

This anti-HMGB1 antibody is a 兔 多克隆 antibody detecting HMGB1 in WB, IF, ICC, IHC (p), IP, IHC (fro) 和 RIP. Suitable for 人.
产品编号 ABIN7464762
发货至: 中国

Quick Overview for HMGB1 抗体 (N-Term) (ABIN7464762)

抗原

See all HMGB1 抗体
HMGB1 (High Mobility Group Box 1 (HMGB1))

适用

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宿主

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克隆类型

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多克隆

标记

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This HMGB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), RNA-Binding Protein Immunoprecipitation (RIP)

质量等级

KO Validated
  • 抗原表位

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    N-Term

    交叉反应

    人, 小鼠, 大鼠

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: mouse brain , rat brain , 293T , HMGB1-transfected 293T

    Validation: KO/KD, Overexpression

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.17 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    HMGB1 (High Mobility Group Box 1 (HMGB1))

    别名

    high mobility group box 1

    背景

    High mobility group box 1 , HMG-1 , HMG1 , HMG3 , SBP-1,DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.

    分子量

    25 kDa

    基因ID

    3146

    UniProt

    P09429

    途径

    p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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