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OCT4 抗体

This anti-OCT4 antibody is a 小鼠 单克隆 antibody detecting OCT4 in WB, IF, ICC, IP, FACS 和 IHC (p). Suitable for 人.
产品编号 ABIN7464699
发货至: 中国

Quick Overview for OCT4 抗体 (ABIN7464699)

抗原

See all OCT4 (POU5F1) 抗体
OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This OCT4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

GT486
  • 交叉反应

    人, 小鼠

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.

    亚型

    IgG2a
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:1000-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: LGNSO transfected HeLa , human ESC

    Validation: KO/KD, Orthogonal, Overexpression

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1.66 mg/mL

    缓冲液

    1XPBS ( pH 7), 20 % Glycerol, No Preservative

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

    别名

    POU class 5 homeobox 1

    背景

    POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4,This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]

    分子量

    39 kDa

    基因ID

    5460

    UniProt

    Q01860

    途径

    Stem Cell Maintenance
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