OCT4 抗体
(4 references)-
- 抗原 See all OCT4 (POU5F1) 抗体
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This OCT4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 交叉反应
- 人, 小鼠, 大鼠
- 产品特性
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Rabbit Polyclonal antibody to OCT4 (POU class 5 homeobox 1)
Oct4 antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 质量等级
- KO Validated
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
- 亚型
- IgG
- Top Product
- Discover our top product POU5F1 Primary Antibody
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anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7-00E-07 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibodyPOU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibodyPOU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 86-119), (N-Term) antibodyPOU5F1 适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB52378 unconjugated
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- 应用备注
- WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: mouse ESC , human ESC , NT2D1
Validation: KO/KD, Orthogonal, Overexpression
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.49 mg/mL
- 缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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: "Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer." in: BMC cancer, Vol. 19, Issue 1, pp. 791, (2020) (PubMed).
: "Increased chemoresistance via Snail-Raf kinase inhibitor protein signaling in colorectal cancer in response to a nicotine derivative." in: Oncotarget, Vol. 7, Issue 17, pp. 23512-20, (2018) (PubMed).
: "Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells." in: Cell death & disease, Vol. 6, pp. e1801, (2016) (PubMed).
: "Sphere-forming-like cells (squamospheres) with cancer stem-like cell traits from VX2 rabbit buccal squamous cell carcinoma." in: International journal of oral science, Vol. 6, Issue 4, pp. 212-8, (2015) (PubMed).
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: "Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer." in: BMC cancer, Vol. 19, Issue 1, pp. 791, (2020) (PubMed).
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- 抗原
- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
- 别名
- POU class 5 homeobox 1 (POU5F1 产品)
- 别名
- OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody
- 背景
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This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6,22)(p21,q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Cellular Localization: Nucleus - 分子量
- 39 kDa
- 基因ID
- 5460
- UniProt
- Q01860
- 途径
- Stem Cell Maintenance
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