LIN28A 抗体
Quick Overview for LIN28A 抗体 (ABIN7464614)
抗原
See all LIN28A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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交叉反应
- 人, 小鼠
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纯化方法
- Affinity purified by Protein G.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human Lin28A. The exact sequence is proprietary.
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亚型
- IgG2a
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anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 108-138) antibody
LIN28A 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB14827 unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (N-Term) antibodyLIN28A 适用: 人, Cow, 犬, Pig, 绵羊 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 93-209) antibodyLIN28A 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 6D1F9 unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 108-138) antibodyLIN28A 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 1-209) antibodyLIN28A 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 168-198), (C-Term) antibodyLIN28A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB14266 unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 177-210), (C-Term) antibodyLIN28A 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB51168 unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 1-209) antibodyLIN28A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (AA 1-209) antibodyLIN28A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Lin-28 Homolog A (C. Elegans) (LIN28A) (C-Term) antibodyLIN28A 适用: 人, 小鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: human ESC , NT2D1 , LIN28 transfected HeLa , mouse placenta
Validation: Overexpression
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, No Preservative
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))
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别名
- lin-28 homolog A
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背景
- Lin-28 homolog A , CSDD1 , LIN-28 , LIN28 , ZCCHC1 , lin-28A,Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
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分子量
- 23 kDa
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基因ID
- 79727
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UniProt
- Q9H9Z2
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途径
- Stem Cell Maintenance
抗原
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