LC3B 抗体 (N-Term)
Quick Overview for LC3B 抗体 (N-Term) (ABIN7464606)
抗原
See all LC3B (MAP1LC3B) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- N-Term
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特异性
- Cross-reactivity may occur with other LC3 isoforms
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交叉反应
- 人, Mosquito, 小鼠, Pig, 大鼠
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: Huh7 (HCV-infected) , Huh7 (3uM-Thapsigargin treatment for 12hr) , mouse brain , Rat brain , NT2D1 , PC-3 , U87-MG , SK-N-SH , HepG2 (3 μM Thapsigargin treatment for 16hr) , HepG2 (3 μM Thapsigargin treatment for 24hr) , HeLa (50μM Chloroquine treatment for 24 hr)
Validation: Comparison, KO/KD, Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.14 mg/mL
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缓冲液
- 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
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别名
- microtubule associated protein 1 light chain 3 beta
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背景
- Microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a,The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]
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分子量
- 15 kDa
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基因ID
- 81631
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UniProt
- Q9GZQ8
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途径
- Autophagy
抗原
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