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LC3B 抗体 (N-Term)

This 兔 多克隆 antibody specifically detects LC3B in WB, IF, IHC (p), FACS, ICC, IP 和 IHC (fro). It exhibits reactivity toward 人.
产品编号 ABIN7464606
发货至: 中国
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Quick Overview for LC3B 抗体 (N-Term) (ABIN7464606)

抗原

See all LC3B (MAP1LC3B) 抗体
LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

适用

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宿主

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克隆类型

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多克隆

标记

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This LC3B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

质量等级

KO Validated
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    N-Term

    特异性

    Cross-reactivity may occur with other LC3 isoforms

    交叉反应

    人, Mosquito, 小鼠, Pig, 大鼠

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: Huh7 (HCV-infected) , Huh7 (3uM-Thapsigargin treatment for 12hr) , mouse brain , Rat brain , NT2D1 , PC-3 , U87-MG , SK-N-SH , HepG2 (3 μM Thapsigargin treatment for 16hr) , HepG2 (3 μM Thapsigargin treatment for 24hr) , HeLa (50μM Chloroquine treatment for 24 hr)

    Validation: Comparison, KO/KD, Orthogonal

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.14 mg/mL

    缓冲液

    1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

    别名

    microtubule associated protein 1 light chain 3 beta

    背景

    Microtubule associated protein 1 light chain 3 beta , ATG8F , LC3B , MAP1A/1BLC3 , MAP1LC3B-a,The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]

    分子量

    15 kDa

    基因ID

    81631

    UniProt

    Q9GZQ8

    途径

    Autophagy
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