Caspase 7 抗体 (C-Term)
Quick Overview for Caspase 7 抗体 (C-Term) (ABIN7464140)
抗原
See all Caspase 7 (CASP7) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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特异性
- IP/MS validation was supported by references (PMID:30377415)
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交叉反应
- 人
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纯化方法
- Purified by antigen-affinity chromatography.
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免疫原
- Recombinant protein encompassing a sequence within the C-terminus region of human Caspase 7. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: 293T , A431 , HeLa , HepG2 , Jurakt (10 μM etoposide treatment for 24 hr)
Validation: IP/MS, KO/KD, Orthogonal
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.93 mg/mL
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缓冲液
- 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))
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别名
- caspase 7
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背景
- Caspase 7 , CASP-7 , CMH-1 , ICE-LAP3 , LICE2 , MCH3,This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms. [provided by RefSeq]
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分子量
- 34 kDa
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基因ID
- 840
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UniProt
- P55210
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
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