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Nanog 抗体

This 小鼠 单克隆 antibody specifically detects Nanog in WB, IF, FACS 和 ICC. It exhibits reactivity toward 人.
产品编号 ABIN7464135
发货至: 中国
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北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Nanog 抗体 (ABIN7464135)

抗原

See all Nanog (NANOG) 抗体
Nanog (NANOG) (Nanog Homeobox (NANOG))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Nanog antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

质量等级

KO Validated

克隆位点

GT3312
  • 交叉反应

    人, 小鼠

    纯化方法

    Affinity purified by Protein G.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.

    亚型

    IgG2b
  • 应用备注

    WB: 1:500-1:3000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    说明

    Positive Control: 293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2

    Validation: KO/KD

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS, No Preservative

    储存液

    Without preservative

    储存条件

    4 °C,-20 °C

    储存方法

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • 抗原

    Nanog (NANOG) (Nanog Homeobox (NANOG))

    别名

    Nanog homeobox

    背景

    Nanog homeobox,Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

    分子量

    35 kDa

    基因ID

    79923

    UniProt

    Q9H9S0

    途径

    Stem Cell Maintenance
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