Nanog 抗体
Quick Overview for Nanog 抗体 (ABIN7464135)
抗原
See all Nanog (NANOG) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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交叉反应
- 人, 小鼠
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纯化方法
- Affinity purified by Protein G.
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免疫原
- Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.
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亚型
- IgG2b
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应用备注
- WB: 1:500-1:3000. FACS: 1:50-1:200. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
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说明
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Positive Control: 293T , A431 , HeLa , HepG2 , human ESC , mouse ESC , Tera-2
Validation: KO/KD
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, No Preservative
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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别名
- Nanog homeobox
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背景
- Nanog homeobox,Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
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分子量
- 35 kDa
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基因ID
- 79923
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UniProt
- Q9H9S0
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途径
- Stem Cell Maintenance
抗原
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