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RNF20 抗体 (AA 125-175)

This anti-RNF20 antibody is a 兔 多克隆 antibody detecting RNF20 in WB, IP 和 IHC (fp). Suitable for 人 和 小鼠.
产品编号 ABIN7454669
发货至: 中国

Quick Overview for RNF20 抗体 (AA 125-175) (ABIN7454669)

抗原

See all RNF20 抗体
RNF20 (Ring Finger Protein 20 (RNF20))

适用

  • 66
  • 35
  • 23
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
人, 小鼠

宿主

  • 59
  • 7

克隆类型

  • 52
  • 14
多克隆

标记

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  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This RNF20 antibody is un-conjugated

应用范围

  • 46
  • 20
  • 13
  • 10
  • 7
  • 5
  • 4
  • 4
  • 3
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
  • 抗原表位

    • 7
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 125-175

    原理

    Rabbit anti-RNF20 Antibody, Affinity Purified

    预测反应

    Chicken,Bovine

    纯化方法

    Affinity Purified

    免疫原

    between AA 125 and 175

    亚型

    IgG
  • 应用备注

    IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    IP: 2 - 10 μg/mg lysate

    WB: 1:2,000 - 1:10,000

    限制

    仅限研究用
  • 浓度

    1000 μg/mL

    缓冲液

    Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    有效期

    12 months
  • 抗原

    RNF20 (Ring Finger Protein 20 (RNF20))

    别名

    RNF20

    背景

    Background: The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription.

    基因ID

    56254

    NCBI登录号

    NP_062538

    UniProt

    Q5VTR2
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