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Topo IIalpha (C-Term) 抗体

The 兔 多克隆 anti-Topo IIalpha antibody (ABIN7454588) specifically detects Topo IIalpha in WB, IHC (fp) 和 IP. The antibody is reactive with 人 samples.
产品编号 ABIN7454588
发货至: 中国
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Quick Overview for Topo IIalpha (C-Term) 抗体 (ABIN7454588)

抗原

Topo IIalpha

适用

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宿主

  • 33
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克隆类型

  • 31
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多克隆

标记

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非结合性

应用范围

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Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)
  • 抗原表位

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    C-Term

    原理

    Rabbit anti-Topo II Alpha Antibody, Affinity Purified

    纯化方法

    Affinity Purified

    免疫原

    between AA 1500 and C-term

    亚型

    IgG
  • 应用备注

    IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

    IP: 2 - 10 μg/mg lysate

    WB: 1:10,000 - 1:30,000

    限制

    仅限研究用
  • 浓度

    1000 μg/mL

    缓冲液

    Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    有效期

    12 months
  • 抗原

    Topo IIalpha

    别名

    Topo II Alpha

    背景

    Background: Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.

    基因ID

    7153

    NCBI登录号

    NP_001058

    UniProt

    P11388
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