LIG1 抗体 (AA 200-250)
Quick Overview for LIG1 抗体 (AA 200-250) (ABIN7454389)
抗原
See all LIG1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 200-250
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原理
- Rabbit anti-DNA Ligase 1 Antibody, Affinity Purified
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纯化方法
- Affinity Purified
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免疫原
- between AA 200 and 250
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亚型
- IgG
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应用备注
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IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
IP: 2 - 5 μg/mg lysate
WB: Not recommended.
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限制
- 仅限研究用
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浓度
- 1000 μg/mL
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缓冲液
- Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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有效期
- 12 months
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- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
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别名
- DNA Ligase 1
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背景
- Background: DNA ligase 1 catalyzes the joining of double-stranded DNA ends during replication, recombination, and repair. Structurally, DNA ligase 1 is toroidal and encircles nicked DNA substrates. The 9-1-1 complex, a DNA damage checkpoint complex, has been reported to associate with DNA ligase 1 and stimulate ligase activity. Mutations in DNA ligase 1 result in immunodeficiency and increased sensitivity to DNA-damaging agents.
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基因ID
- 3978
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NCBI登录号
- NP_000225
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UniProt
- P18858
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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