PLAP 抗体
Quick Overview for PLAP 抗体 (ABIN7448143)
抗原
See all PLAP (ALPP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
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交叉反应 （详细）
- Human.
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纯化方法
- 200 µg/mL of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human ALPP protein
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亚型
- IgG1
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anti-Placental Alkaline Phosphatase (ALPP) (AA 162-312) antibody
ALPP 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 小鼠 Monoclonal ALP-870 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (N-Term) antibodyALPP 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 小鼠 Monoclonal GM022 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 兔 Monoclonal ALPP-2899R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-350R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (N-Term) antibodyALPP 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (AA 20-143) antibodyALPP 适用: 人 WB, ELISA, IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (AA 534-792) antibodyALPP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: JEG-3 or HepG2 cells. Human placenta or seminoma.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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别名
- ALPP
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背景
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Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP1,Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)
Cellular localisation: Cytoplasmic. Cell surface. -
分子量
- 70kDa
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基因ID
- 250, 284255
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UniProt
- P05187
抗原
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