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Recombinant GFP 抗体 (Biotin)

Name: Recombinant GFP 抗体 (Biotin)
SKU: ABIN7272885
Availability: OutOfStock

GFP 适用: Aequorea victoria, Aequorea aequorea IF, ICC, FACS 宿主: Alpaca Monoclonal 1H1 Biotin single-domain Antibody (sdAb) Recombinant Antibody

DNA PAINT image of nucelar pore complexes using the DBCO conjugated version of this single domain anti-GFP antibody modified with DNA oligos. (Recombinant GFP 抗体 (Biotin))

SRM

1 image

产品编号 ABIN7272885

发货至: 中国

Datasheet as PDF

抗原 See all GFP 抗体

GFP (Green Fluorescent Protein (GFP))

抗体类型

Recombinant Antibody

片段

single-domain Antibody (sdAb)
适用
157

19

15

15

11

7

5

4

2

1

1

1

1
Aequorea victoria, Aequorea aequorea
宿主
67

66

35

10

8

5

1

1
Alpaca

Expression System

E.coli
克隆类型
115

73
单克隆
标记
95

11

10

7

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Biotin
应用范围
158

75

46

36

31

26

26

21

16

14

14

13

12

6

4

4

3

2

1

1

1

1

1

1
Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

特异性

GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus, mClover3 and Citrine

纯化方法

Produced in: E.coli

免疫原

Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria

克隆位点

1H1

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应用备注

Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconsitute with 250 µL 50% glycerol for storage at -80 °C

缓冲液

sdAbs are lyophilized from PBS pH 7.4.

储存条件

4 °C/-20 °C

储存方法

Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
抗原

GFP (Green Fluorescent Protein (GFP))

别名

GFP (GFP 产品)

别名

green fluorescent protein antibody, gfp antibody

背景

A breakthrough in the field of biology and bioluminescence started in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants here.