Digoxigenin 抗体
The 小鼠 单克隆 anti-Digoxigenin antibody (Clone 21H8) (ABIN7233195) specifically detects Digoxigenin in ELISA, WB 和 IHC.
The antibody is reactive with Digitalis purpurea (Common foxglove) samples.
产品编号 ABIN7233195
发货至:
中国
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Quick Overview for Digoxigenin 抗体 (ABIN7233195)
抗原
Digoxigenin (DIG)
适用
Digitalis purpurea (Common foxglove)
宿主
小鼠
克隆类型
单克隆
标记
This Digoxigenin antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
克隆位点
21H8
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原理
- Anti-Digoxigenin Mouse Monoclonal Antibody
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特异性
- This antibody reacts with free and bound digoxigenin.
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交叉反应
- Digitalis purpurea (Common foxglove)
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免疫原
- Digoxigenin conjugate.
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亚型
- IgG1
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应用备注
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ELISA: use at 0.1-1.0 μg/mL
Immunoblotting:
User should use at 2-5 μg/mL.
Immunohistochemistry: Use at 1-5 μg/mL.
These are recommended concentrations.
End user should determine optimal concentrations for their applications. -
限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 μg/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
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缓冲液
- 0.1M Tris, 0.1M glycine, 2 % sucrose
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储存条件
- -20 °C,-80 °C
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储存方法
- This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
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- Digoxigenin (DIG)
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别名
- Digoxigenin
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物质类
- Chemical
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背景
- Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea, Digitalis orientalis and Digitalis lanata (foxgloves). It is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein and is often used as an immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically uridine), which is then incorporated into RNA as it is synthesized.
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CAS-编号
- 1672-46-4
抗原
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