Digoxigenin 抗体
Cited in 1 publication.
The 绵羊 多克隆 anti-Digoxigenin antibody (ABIN2473338) specifically detects Digoxigenin in ELISA, WB 和 IHC (fro).
The antibody is reactive with 化学剂 samples.
Independently validated for use in Immunohistochemistry.
(1 reference)
(1 validation)
产品编号 ABIN2473338
发货至:
中国
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Quick Overview for Digoxigenin 抗体 (ABIN2473338)
抗原
Digoxigenin (DIG)
适用
化学剂
宿主
绵羊
克隆类型
多克隆
标记
This Digoxigenin antibody is un-conjugated
应用范围
ELISA, Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
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产品特性
- Purified IgG
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纯化方法
- Purified
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免疫原
- Digoxin-3-CM conjugated to keyhole limpet haemocyanin
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亚型
- IgG
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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- by
- Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
- No.
- #104646
- 日期
- 2026.06.08
- 抗原
- Digoxigenin (DIG)
- Lot Number
- 171281
- Method validated
- Immunohistochemistry
- Positive Control
Sections from the same tissues were incubated with an anti-digoxigenin antibody conjugated with alkaline phosphatase (AP) and developed with NBT/BCIP solution.
- Negative Control
Omission of Terminal transferase.
- Notes
The primary antibody works in IHC-P at concentrations from 1:200-1:500. The secondary antibody works in IHC-P at concentration of 1:1000.
- Primary Antibody
- ABIN2473338
- Secondary Antibody
- ABIN102261 – Rabbit anti-Sheep IgG (Heavy & Light Chain) Antibody (FITC) – Preadsorbed (Rockland Immunochemicals, Inc.), lot# 27867
- Full Protocol
- Sample analyzed: P5 CD1 mouse brain
- Paraffin-embedded slices (7 µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O).
- Sections were incubated in proteinase K [20 µg/mL] for 15 minutes at room temperature (RT).
- 4 × 2 minutes washing in double distilled water (ddH2O).
- Sections were briefly washed in DN buffer and incubated in DNase [1 µg/mL] for 10 minutes at RT.
- 4 × 2 minutes washing in ddH2O.
- 5 minutes washing in TDT buffer at RT.
- Sections were incubated in TDT buffer added with Terminal transferase [0.05 U/µL] and digoxigenin-11-dUTP [10 µM] for 2 hours at +37 °C.
- 15 minutes washing in TB buffer at RT.
- 5 minutes washing in ddH2O + 5 minutes washing in 0.01 M PBS.
- Sections were incubated with the primary antibody at the following dilutions of 1:50, 1:200, 1:500, overnight at +4 °C (primary antibody was diluted in PLL/BSA/PBS diluent added with 5% BSA).
- 3 × 5-minute washes in 0.01 M PBS.
- Incubation with the anti-mouse secondary antibody 1:1000 in PLL/BSA/PBS diluent, 1 hour at RT.
- 3 × 5-minute washes in 0.01 M PBS.
- Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) [1 µg/mL], 2 minutes.
- 2 × 5 minutes washing in 0.01 M PBS.
- Specimens were mounted in Fluoroshield mounting medium (Sigma).
- Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 40× magnification.
OTHER PERTINENT REAGENTS USED:
- Proteinase K (Thermo Scientific, Catalog # EO0491)
- DNase I (in the kit by Sigma, Catalog # AMP-D1)
- Terminal transferase (in the kit by Jena Biosciences, Catalog # APP-002)
- Digoxigenin-11-dUTP (Roche, Catalog # 11573179910)
- DN buffer: Tris base [30 mM] pH 7.2, sodium cacodylate [140 mM], magnesium chloride [4 mM], DTT [0.1 mM] in dH2O
- TDT buffer: Tris base [30 mM] pH 7.2, sodium cacodylate [140 mM], cobalt chloride [1 mM] in dH2O
- TB buffer: sodium chloride [300 mM], sodium citrate [30 mM] in dH2O
- Experimental Notes
To exclude any nonspecific signal due to the secondary antibody, one control slice was processed for each experimental procedure, omitting the primary antibody.
生效 #104646 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Positive control with Alkaline Phosphatase Anti-Digoxigenin antibody on P5 CD1 mouse brain. Images show reaction with (positive control) and without (TUNEL reaction) DNase treatment. Magnification: 40x.]()
Positive control with Alkaline Phosphatase Anti-Digoxigenin antibody on P5 CD1 mouse brain. Images show reaction with (positive control) and without (TUNEL reaction) DNase treatment. Magnification: 40x.
![Digoxigenin (DIG) immunoreactive cells in the cerebral cortex, hippocampus and cerebellum of P5 CD1 mice. Sections were treated with DNase and subjected to TUNEL reaction. Magnification: 40x.]()
Digoxigenin (DIG) immunoreactive cells in the cerebral cortex, hippocampus and cerebellum of P5 CD1 mice. Sections were treated with DNase and subjected to TUNEL reaction. Magnification: 40x.
Full Methods -
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状态
- Liquid
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浓度
- 5 mg/mL
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: "The perivascular space as a path of hematopoietic progenitor cells and mature T cells between the blood circulation and the thymic parenchyma." in: International immunology, Vol. 19, Issue 6, pp. 745-53, (2007) (PubMed).
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- Digoxigenin (DIG)
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别名
- DIGOXIGENIN
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物质类
- Chemical
抗原
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