ATG12 抗体 (C-Term)
Quick Overview for ATG12 抗体 (C-Term) (ABIN7232888)
抗原
See all ATG12 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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原理
- Anti-Atg12 Rabbit Polyclonal Antibody
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特异性
- This antibody recognizes human Atg12.
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交叉反应
- 人
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免疫原
- Synthetic peptide corresponding to amino acids at the C-terminus of human Atg12.
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anti-Autophagy Related 12 (ATG12) (N-Term) antibody
ATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 猴, 小鸡, 兔, Pig, Cat, 驴, 山羊, Hamster, 绵羊, Avian WB, IF 宿主: 山羊 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) antibodyATG12 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal 43CT73-3-5-5-4 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 101-187) antibodyATG12 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-74) antibodyATG12 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-30), (N-Term) antibodyATG12 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7475 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-50) antibodyATG12 适用: 人, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) antibodyATG12 适用: 人 WB, IHC, IP 宿主: 兔 Monoclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 79-128) antibodyATG12 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 斑马鱼, 猴, 小鸡, 兔, Pig, Bat, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-74) antibodyATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H8 unconjugated
anti-Autophagy Related 12 (ATG12) (AA 1-140) antibodyATG12 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F9 unconjugated
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应用备注
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Immunoblotting: use at 1-2 μg/mL. Bands of ~55 kDa, corresponding to Atg12-Atg5 complex, and ~15kda, corresponding to free Atg12, are detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications. -
限制
- 仅限研究用
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状态
- Liquid
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溶解方式
- Dilute in PBS or medium that is identical to that used in the assay system.
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- This antibody is stable for at least one (1) year at -20°C.
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- ATG12 (Autophagy Related 12 (ATG12))
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别名
- ATG12
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背景
- Ubiquitin-like protein ATG12,Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. {PubMed:12207896, PubMed:17999726, PubMed:19074260, PubMed:19164948, PubMed:23202584}., (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601). {PubMed:17709747, PubMed:19666601}.,Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Atg12 is an ubiquitin-like protein involved in autophagy vesicle formation. Conjugation with Atg5 through a ubiquitin-like conjugating system involving also Atg7 as an E1-like activating enzyme and Atg10 as an E2-like conjugating enzyme, is essential for its function. The Atg12-Atg5 conjugate acts as an E3-like enzyme which is required for lipidation of Atg8 family proteins and their association to vesicle membranes. The Atg12-Atg5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS.,Cytoplasm, Preautophagosomal structure membrane,Autophagy-related protein 12, APG12-like
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UniProt
- O94817
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途径
- Autophagy
抗原
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