Vimentin 抗体 (pTyr61)
Quick Overview for Vimentin 抗体 (pTyr61) (ABIN7224922)
抗原
See all Vimentin (VIM) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr61
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原理
- Vimentin (phospho Tyr61) Polyclonal Antibody
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特异性
- Phospho-Vimentin (Y61) Polyclonal Antibody detects endogenous levels of Vimentin protein only when phosphorylated at Y61.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Vimentin Phospho-Tyr61
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Vimentin (VIM)
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别名
- Vimentin
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背景
- Rabbit Anti-Vimentin (phospho Tyr61) Polyclonal Antibody,VIM, Vimentin,VIM (vimentin) encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in VIM causes a dominant, pulverulent cataract.,Vimentin
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分子量
- observerd band 57kDa
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基因ID
- 7431
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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