TFEB 抗体 (AA 10-90)
Quick Overview for TFEB 抗体 (AA 10-90) (ABIN7225064)
抗原
See all TFEB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 10-90
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原理
- TFEB Polyclonal Antibody
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特异性
- TFEB Polyclonal Antibody detects endogenous levels of TFEB protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the N-terminal region of human TFEB at AA range: 10-90
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亚型
- IgG
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anti-Transcription Factor EB (TFEB) (AA 1-476) antibody
TFEB 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal S1 unconjugated
anti-Transcription Factor EB (TFEB) (Middle Region) antibodyTFEB 适用: 人, Cow, 犬, 马, 山羊, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor EB (TFEB) (AA 1-476) antibodyTFEB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor EB (TFEB) (AA 1-220) antibodyTFEB 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor EB (TFEB) (N-Term) antibodyTFEB 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor EB (TFEB) (N-Term) antibodyTFEB 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor EB (TFEB) (Internal Region) antibodyVerified TFEB 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Transcription Factor EB (TFEB) (AA 1-476) antibodyTFEB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Transcription Factor EB (TFEB) (C-Term) antibodyTFEB 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig, 马, 兔, 豚鼠, 猴, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- TFEB (Transcription Factor EB (TFEB))
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别名
- TFEB
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背景
- Rabbit Anti-TFEB Polyclonal Antibody,TFEB, BHLHE35, Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35,Transcription factor EB (TFEB) is a member of the Myc-related, bHLH leucine-zipper family of transcription factors that drives the expression of a network of genes known as the Coordinated Lysosomal Expression and Regulation (CLEAR) network. TFEB specifically recognizes and binds regulatory sequences within the CLEAR box (GTCACGTGAC) of lysosomal and autophagy genes, resulting in the up-regulated expression of genes involved in lysosome biogenesis and function, and regulation of autophagy. TFEB is activated in response to nutrient deprivation, stimulating translocation to the nucleus where it forms homo- or heterooligomers with other members of the microphthalmia transcription factor (MiTF) subfamily and resulting in up-regulation of autophagosomes and lysosomes. Recently, it has been shown that TFEB is a component of mammalian target of rapamycin (mTOR) complex 1 (mTORC1), which regulates the phosphorylation and nuclear translocation of TFEB in response to cellular starvation and stress. During normal growth conditions, TFEB is phosphorylated at Ser211 in an mTORC1-dependent manner. Phosphorylation promotes association of TFEB with 14-3-3 family proteins and retention in the cytosol. Inhibition of mTORC1 results in a loss of TFEB phosphorylation, dissociation of the TFEB/14-3-3 complex, and rapid transport of TFEB to the nucleus where it increases transcription of CLEAR and autophagy genes. TFEB has also been shown to be activated in a nutrient-dependent manner by p42 MAP kinase (Erk2). TFEB is phosphorylated at Ser142 by Erk2 in response to nutrient deprivation, resulting in nuclear localization and activation, and indicating that pathways other than mTOR contribute to nutrient sensing via TFEB.,Transcription factor EB
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分子量
- observerd band 52kDa
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基因ID
- 7942
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UniProt
- P19484
抗原
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