RFWD3 抗体 (AA 350-430)
Quick Overview for RFWD3 抗体 (AA 350-430) (ABIN7223390)
抗原
See all RFWD3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 350-430
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原理
- RFWD3 Polyclonal Antibody
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特异性
- RFWD3 Polyclonal Antibody detects endogenous levels of RFWD3 protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the Internal region of human RFWD3 at AA range: 350-430
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亚型
- IgG
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anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (N-Term) antibody
RFWD3 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (Internal Region) antibodyRFWD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 3 (RFWD3) (AA 651-749) antibodyRFWD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B4 unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- RFWD3 (Ring Finger and WD Repeat Domain 3 (RFWD3))
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别名
- RFWD3
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背景
- Rabbit Anti-RFWD3 Polyclonal Antibody,RFWD3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201,RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53. (PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.,E3 ubiquitin-protein ligase RFWD3
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分子量
- observerd band 85kDa
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基因ID
- 55159
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UniProt
- Q6PCD5
抗原
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