RAD17 抗体 (pSer645)
Our Local Distributor
北京 101111
Quick Overview for RAD17 抗体 (pSer645) (ABIN7213160)
抗原
See all RAD17 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- pSer645
-
原理
- Rad17 (phospho Ser645) Polyclonal Antibody
-
特异性
- Phospho-Rad17 (S645) Polyclonal Antibody detects endogenous levels of Rad17 protein only when phosphorylated at S645.
-
纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
-
免疫原
- Synthesized peptide derived from human Rad17 Phospho-Ser645
-
亚型
- IgG
-
-
anti-RAD17 (RAD17) (AA 1-584) antibody
RAD17 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) (AA 391-670) antibodyRAD17 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) (AA 1-110) antibodyRAD17 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-RAD17 (RAD17) (AA 1-270) antibodyRAD17 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) (C-Term) antibodyRAD17 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) antibodyKD Validated RAD17 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) (C-Term) antibodyRAD17 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) (C-Term) antibodyRAD17 适用: 人, 小鼠, 大鼠, 犬, Cow, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) (AA 621-670) antibodyRAD17 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAD17 (RAD17) antibodyRAD17 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 3-10E-010 unconjugated
-
-
应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.
-
说明
-
Primary Antibody
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
-
-
- RAD17
-
别名
- Rad17
-
背景
- Rabbit Anti-Rad17 (phospho Ser645) Polyclonal Antibody,RAD17, R24L, Cell cycle checkpoint protein RAD17, hRad17, RF-C/activator 1 homolog,RAD17 checkpoint clamp loader component encoded by RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. RAD17 checkpoint clamp loader component shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. RAD17 checkpoint clamp loader component binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. RAD17 checkpoint clamp loader component recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of RAD17 checkpoint clamp loader component is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.,Cell cycle checkpoint protein RAD17
-
分子量
- observerd band 77kDa
-
基因ID
- 5884
-
UniProt
- O75943
抗原
-
