NFYA 抗体 (AA 230-310)
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Quick Overview for NFYA 抗体 (AA 230-310) (ABIN7216046)
抗原
See all NFYA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 230-310
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原理
- NF-YA Polyclonal Antibody
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特异性
- NF-YA Polyclonal Antibody detects endogenous levels of NF-YA protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from the C-terminal region of human NF-YA at AA range: 230-310
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亚型
- IgG
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- NFYA (Nuclear Transcription Factor Y, alpha (NFYA))
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别名
- NF-YA
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背景
- Rabbit Anti-NF-YA Polyclonal Antibody,NFYA, Nuclear transcription factor Y subunit alpha, CAAT box DNA-binding protein subunit A, Nuclear transcription factor Y subunit A, NF-YA,The protein encoded by NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in NFYA product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms.,Nuclear transcription factor Y subunit alpha
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分子量
- observerd band 43kDa
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基因ID
- 4800
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UniProt
- P23511
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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