IkappaB-epsilon 抗体 (pSer22)
This anti-IkappaB-epsilon antibody is a 兔 多克隆 antibody detecting IkappaB-epsilon in IF, ELISA, WB 和 IHC (p). Suitable for 人, 小鼠 和 大鼠.
Quick Overview for IkappaB-epsilon 抗体 (pSer22) (ABIN7222756)
抗原
IkappaB-epsilon
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This IkappaB-epsilon antibody is un-conjugated
应用范围
Immunofluorescence (IF), ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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抗原表位
- pSer22
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原理
- IκB-ε (phospho Ser22) Polyclonal Antibody
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特异性
- Phospho-IκB-ε (S22) Polyclonal Antibody detects endogenous levels of IκB-ε protein only when phosphorylated at S22.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human IkappaB-epsilon Phospho-Ser22
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亚型
- IgG
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anti-IkappaB-epsilon (AA 131-180), (pSer22) antibody
适用: 人, 小鼠, 大鼠 IF, ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- IkappaB-epsilon
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背景
- Rabbit Anti-IκB-ε (phospho Ser22) Polyclonal Antibody,NFKBIE, IKBE, NF-kappa-B inhibitor epsilon, NF-kappa-BIE, I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon,The protein encoded by NFKBIE binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus.,NF-kappa-B inhibitor epsilon
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分子量
- observerd band 38kDa
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基因ID
- 4794
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UniProt
- O00221
抗原
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