IkappaB-epsilon 抗体 (pSer22)
The 兔 多克隆 anti-IkappaB-epsilon antibody (ABIN1531184) specifically detects IkappaB-epsilon in IF, IHC, ELISA 和 WB.
The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN1531184
发货至:
中国
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Contact Info
Our Local Distributor
中国
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北京 101111
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Quick Overview for IkappaB-epsilon 抗体 (pSer22) (ABIN1531184)
抗原
IkappaB-epsilon
适用
人, 小鼠, 大鼠
宿主
兔
克隆类型
多克隆
标记
This IkappaB-epsilon antibody is un-conjugated
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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抗原表位
- AA 131-180, pSer22
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原理
- IκB-ε (phospho Ser22) Antibody
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特异性
- Phospho-IκB-ε (S22) Polyclonal Antibody detects endogenous levels of IκB-ε protein only when phosphorylated at S22.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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纯度
- > 95 %
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免疫原
- The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22. AA range:131-180
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亚型
- IgG
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应用备注
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20°C/1 year
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有效期
- 12 months
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- IkappaB-epsilon
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背景
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Synonyms: NFKBIE, IKBE, NF-kappa-B inhibitor epsilon, NF-kappa-BIE, I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon
Background: The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. [provided by RefSeq, Sep 2011],
Gene Name: NFKBIE
Protein Name: NF-kappa-B inhibitor epsilon
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分子量
- 38 kDa
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基因ID
- 4794
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OMIM
- 604548
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UniProt
- O00221
抗原
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