Separase 抗体
Quick Overview for Separase 抗体 (ABIN7216974)
抗原
See all Separase (ESPL1) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Separase Polyclonal Antibody
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特异性
- Separase Polyclonal Antibody detects endogenous levels of Separase protein.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Separase around the non-phosphorylation site of S801
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亚型
- IgG
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anti-Extra Spindle Poles Like 1 (ESPL1) (AA 586-701) antibody
ESPL1 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) antibodyESPL1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (AA 586-702) antibodyESPL1 适用: 人 ELISA, IHC, WB, IHC (p) 宿主: 小鼠 Monoclonal 6H6 unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (Tyr489) antibodyESPL1 适用: 人, 小鼠 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer1126) antibodyESPL1 适用: 人 IHC (p), DB 宿主: 兔 Polyclonal RB7943 unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer801) antibodyESPL1 适用: 人 IHC, WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (AA 767-816) antibodyESPL1 适用: 人, 小鼠 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (pSer801) antibodyESPL1 适用: 人 ELISA, IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (AA 1220-1500) antibodyESPL1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Extra Spindle Poles Like 1 (ESPL1) (AA 1292-1495) antibodyESPL1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:40000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))
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别名
- Separase
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背景
- Rabbit Anti-Separase Polyclonal Antibody,ESPL1, ESP1, KIAA0165, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase,Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21, MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.,Separin
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分子量
- observerd band 230kDa
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基因ID
- 9700
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UniProt
- Q14674
抗原
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