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EIF4E 抗体 (pSer209)

The 兔 多克隆 anti-EIF4E antibody is suitable to detect EIF4E in samples from 人, 大鼠 和 小鼠. It has been validated for WB, IHC, ELISA 和 IF.
产品编号 ABIN7212948
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EIF4E 抗体 (pSer209) (ABIN7212948)

抗原

See all EIF4E 抗体
EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

适用

  • 153
  • 92
  • 80
  • 20
  • 5
  • 5
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  • 3
  • 2
  • 2
  • 2
  • 2
人, 大鼠, 小鼠

宿主

  • 133
  • 20
  • 2

克隆类型

  • 99
  • 56
多克隆

标记

  • 69
  • 11
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  • 5
  • 4
  • 4
  • 4
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  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
This EIF4E antibody is un-conjugated

应用范围

  • 134
  • 70
  • 50
  • 42
  • 38
  • 34
  • 33
  • 27
  • 26
  • 15
  • 7
  • 6
  • 2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)
  • 抗原表位

    • 54
    • 15
    • 8
    • 7
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer209

    原理

    Rabbit Anti-eIF4E (phospho Ser209) Polyclonal Antibody

    特异性

    Phospho-eIF4E (S209) Polyclonal Antibody detects endogenous levels of eIF4E protein only when phosphorylated at S209.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    免疫原

    Synthesized peptide derived from human eIF4E Phospho-Ser209

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    有效期

    12 months
  • 抗原

    EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

    别名

    eIF4E

    背景

    EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding proteinEukaryotic translation initiation factor 4E encoded by EIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs.Eukaryotic translation initiation factor 4E aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. EIF4E acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of EIF4E are found on other chromosomes. Alternative splicing results in multiple transcript variants.

    分子量

    25kD

    基因ID

    1977

    UniProt

    P06730

    途径

    BCR Signaling
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