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EIF2A 抗体 (pSer51)

This 兔 多克隆 anti-EIF2A antibody specifically detects EIF2A in WB, IF, ELISA 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7203754
发货至: 中国
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Quick Overview for EIF2A 抗体 (pSer51) (ABIN7203754)

抗原

See all EIF2A 抗体
EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

适用

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人, 小鼠, 大鼠

宿主

  • 98
  • 15
  • 2

克隆类型

  • 66
  • 49
多克隆

标记

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This EIF2A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 17
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    pSer51

    原理

    eIF2α (phospho Ser51) Polyclonal Antibody

    特异性

    Phospho-eIF2α (S51) Polyclonal Antibody detects endogenous levels of eIF2α protein only when phosphorylated at S51.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

    免疫原

    Synthesized peptide derived from human eIF2alpha Phospho-Ser51

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IF (1:50-1:200), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
  • 抗原

    EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

    别名

    eIF2alpha

    背景

    Rabbit Anti-eIF2α (phospho Ser51) Polyclonal Antibody,EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha,The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2, MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).,Eukaryotic translation initiation factor 2 subunit 1

    基因ID

    1965

    UniProt

    P05198

    途径

    Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
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