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TdT 抗体 (AA 350-430)

This 兔 多克隆 antibody specifically detects TdT in WB, ELISA, IHC 和 IF. It exhibits reactivity toward 人 和 小鼠.
产品编号 ABIN7220066
发货至: 中国
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Quick Overview for TdT 抗体 (AA 350-430) (ABIN7220066)

抗原

See all TdT (DNTT) 抗体
TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

适用

  • 96
  • 30
  • 28
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
人, 小鼠

宿主

  • 82
  • 17

克隆类型

  • 62
  • 36
  • 1
多克隆

标记

  • 53
  • 7
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
This TdT antibody is un-conjugated

应用范围

  • 64
  • 40
  • 32
  • 9
  • 8
  • 8
  • 4
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

    • 12
    • 8
    • 8
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 350-430

    原理

    Rabbit Anti-TdT Polyclonal Antibody

    特异性

    TdT Polyclonal Antibody detects endogenous levels of TdT protein.

    纯化方法

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

    免疫原

    Synthesized peptide derived from the Internal region of human TdT at AA range: 350-430

    亚型

    IgG
  • 应用备注

    Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:40000,IF 1:50-200

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

    有效期

    12 months
  • 抗原

    TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

    别名

    TdT

    背景

    DNTT, TDT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferaseDNTT is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

    分子量

    60kD

    基因ID

    1791

    UniProt

    P04053

    途径

    DNA Damage Repair
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