Cyclin D1 抗体 (pThr286)
Quick Overview for Cyclin D1 抗体 (pThr286) (ABIN7213162)
抗原
See all Cyclin D1 (CCND1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr286
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原理
- Cyclin D1 (phospho Thr286) Polyclonal Antibody
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特异性
- Phospho-Cyclin D1 (T286) Polyclonal Antibody detects endogenous levels of Cyclin D1 protein only when phosphorylated at T286.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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免疫原
- Synthesized peptide derived from human Cyclin D1 Phospho-Thr286
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亚型
- IgG
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anti-Cyclin D1 (CCND1) (AA 61-110) antibody
CCND1 适用: 人, 小鼠, 大鼠, Cow, 兔, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) (AA 167-295) antibodyCCND1 适用: 人 IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3B6G3 unconjugated
anti-Cyclin D1 (CCND1) (AA 1-295) antibodyCCND1 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1F7F5 unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) antibodyKD Validated CCND1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB735 unconjugated Recombinant Antibody
anti-Cyclin D1 (CCND1) (AA 68-97) antibodyCCND1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB8066 unconjugated
anti-Cyclin D1 (CCND1) (C-Term) antibodyCCND1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cyclin D1 (CCND1) antibodyCCND1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal DCS-6 unconjugated
anti-Cyclin D1 (CCND1) (AA 1-295) antibodyCCND1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.
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说明
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Primary Antibody
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
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- Cyclin D1 (CCND1)
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别名
- Cyclin D1
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背景
- Rabbit Anti-Cyclin D1 (phospho Thr286) Polyclonal Antibody,CCND1, BCL1, PRAD1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene,Cyclin D1 encoded by CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. Cyclin D1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of CCND1, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.,G1
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分子量
- observerd band 33kDa
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基因ID
- 595
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UniProt
- P24385
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途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
抗原
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