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Cyclin D1 抗体 (AA 61-110)

Name: Cyclin D1 抗体 (AA 61-110)
SKU: ABIN671016
Availability: OutOfStock

CCND1 适用: 人, 大鼠, 小鼠, Cow, 兔, 绵羊 WB, ELISA, FACS, IHC (p), IF (cc), IHC (fro), IF (p) 宿主: 兔 Polyclonal unconjugated

A549 cells probed with Anti-Cyclin D1 Polyclonal Antibody at 1:5000 90min in 37˚C.

Formalin-fixed and paraffin embedded human placenta tissue labeled with Anti-Cyclin D1 Polyclonal Antibody, Unconjugated (ABIN671016) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

RSC96 cells probed with Cyclin D1 Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

Image provided by One World Lab validation program.

IF (cc)

6 images

(15 references)

产品编号 ABIN671016

发货至: 中国

Datasheet as PDF

抗原 See all Cyclin D1 (CCND1) 抗体

Cyclin D1 (CCND1)
抗原表位
22

21

16

15

13

11

10

9

7

7

7

7

7

6

6

5

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 61-110
适用
241

127

123

37

19

18

16

10

9

8

4

3

3

3

3

2

1

1

1

1

1
人, 大鼠, 小鼠, Cow, 兔, 绵羊
宿主
209

56

1
兔
Compatible Secondaries
克隆类型
198

66
多克隆
标记
117

29

21

9

8

7

7

7

7

7

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1
This Cyclin D1 antibody is un-conjugated
应用范围
202

88

70

65

42

31

26

26

24

22

19

12

6

5

3

2

2

2

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

Cow, 人, 小鼠, 兔, 大鼠, 绵羊

预测反应

Dog

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from mouse Cyclin D1

亚型

IgG
anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 96-295) antibody
CCND1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
KO Validated CCND1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 167-295) antibody
CCND1 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 3B6G3 unconjugated

anti-Cyclin D1 (CCND1) (AA 1-295) antibody
CCND1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1F7F5 unconjugated

anti-Cyclin D1 (CCND1) (C-Term) antibody
CCND1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB8066 unconjugated

anti-Cyclin D1 (CCND1) (AA 68-97) antibody
CCND1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cyclin D1 (CCND1) antibody
CCND1 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal DCS-6 unconjugated

anti-Cyclin D1 (CCND1) (AA 1-233) antibody
CCND1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Jiang, Xie, Cai, Ren, Ma: "Effects of hepatitis C virus core protein and nonstructural protein 4B on the Wnt/β-catenin pathway." in: BMC microbiology, Vol. 17, Issue 1, pp. 124, (2017) (PubMed).

Wang, Cao, Sun, Dai, Fang, Li, Yan, Mao, Zhang: "Total flavonoid aglycones extract in Radix scutellariae inhibits lung carcinoma and lung metastasis by affecting cell cycle and DNA synthesis." in: Journal of ethnopharmacology, Vol. 194, pp. 269-279, (2016) (PubMed).

Zhang, Pan, Xu, Niu, Ma, Xu: "Interleukin 18 augments growth ability via NF-?B and p38/ATF2 pathways by targeting cyclin B1, cyclin B2, cyclin A2, and Bcl-2 in BRL-3A rat liver cells." in: Gene, Vol. 563, Issue 1, pp. 45-51, (2015) (PubMed).

Wang, Yang, Liu, Huang, Wang, Chen, Chen: "RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway." in: Biochemical and biophysical research communications, Vol. 454, Issue 1, pp. 71-7, (2015) (PubMed).

Yang, Wang, Hui, Li, Jiang: "SOX 1, contrary to SOX 2, suppresses proliferation, migration, and invasion in human laryngeal squamous cell carcinoma by inhibiting the Wnt/?-catenin pathway." in: Tumour biology, (2015) (PubMed).

Shi, Lu, Zhou, Tan, He, Ding, Tian, Wang, Wang: "Long non-coding RNA Loc554202 regulates proliferation and migration in breast cancer cells." in: Biochemical and biophysical research communications, Vol. 446, Issue 2, pp. 448-53, (2014) (PubMed).

Ye, Li, Li, Yang, Niu, Wang: "Anti-tumor activity and relative mechanism of ethanolic extract of Marsdenia tenacissima (Asclepiadaceae) against human hematologic neoplasm in vitro and in vivo." in: Journal of ethnopharmacology, Vol. 153, Issue 1, pp. 258-67, (2014) (PubMed).

Lin, Wang, Wang, Fang, Zhang, Yang, Guo, Lin, Zhang, Wang: "Lithocarpus polystachyus REHD leaf aqueous extract inhibits human breast cancer growth in vitro and in vivo." in: Nutrition and cancer, Vol. 66, Issue 4, pp. 613-24, (2014) (PubMed).

He, Xiang, Zong, Yan, Yu, Liu, Zou, Yang: "CDK2-AP1 inhibits growth of breast cancer cells by regulating cell cycle and increasing docetaxel sensitivity in vivo and in vitro." in: Cancer cell international, Vol. 14, Issue 1, pp. 130, (2014) (PubMed).

Souza, Silva, Calloway, Kucuk, Rossi, McLemore: "Genistein protects hematopoietic stem cells against G-CSF-induced DNA damage." in: Cancer prevention research (Philadelphia, Pa.), Vol. 7, Issue 5, pp. 534-44, (2014) (PubMed).

Li, Yu, Liu, Zhu, He, Peng, Hua: "miR-34c works downstream of p53 leading to dairy goat male germline stem-cell (mGSCs) apoptosis." in: Cell proliferation, Vol. 46, Issue 2, pp. 223-31, (2013) (PubMed).

Teng, Chang, Wang, Lu, Wu, Yang, Chiu, Yang, Hsu, Ho: "Phospholipid-functionalized mesoporous silica nanocarriers for selective photodynamic therapy of cancer." in: Biomaterials, Vol. 34, Issue 30, pp. 7462-70, (2013) (PubMed).

Wang, Wu, Gao, Chen, Gu, Ding, Guo: "Elevated toll-like receptor 3 inhibits pancreatic ?-cell proliferation through G1 phase cell cycle arrest." in: Molecular and cellular endocrinology, Vol. 377, Issue 1-2, pp. 112-22, (2013) (PubMed).

Zhang, Tan, Wang, Chen, Quan, Xian, Zhang, He, Zhang: "Musashi2 modulates K562 leukemic cell proliferation and apoptosis involving the MAPK pathway." in: Experimental cell research, Vol. 320, Issue 1, pp. 119-27, (2013) (PubMed).

Chen, Shi, Liu, Fang, Zhang, Zhao: "Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis." in: BMC cancer, Vol. 12, pp. 464, (2012) (PubMed).
抗原

Cyclin D1 (CCND1)

别名

Cyclin D1 (CCND1 产品)

别名

BCL1 antibody, D11S287E antibody, PRAD1 antibody, U21B31 antibody, AI327039 antibody, CycD1 antibody, Cyl-1 antibody, bcl-1 antibody, cD1 antibody, cb161 antibody, cycd1 antibody, etID37810.7 antibody, fb52e01 antibody, fc45c08 antibody, fc83a12 antibody, id:ibd1198 antibody, wu:fb52e01 antibody, wu:fc45c08 antibody, wu:fc83a12 antibody, ccnd1b antibody, prad1 antibody, ccnd1 antibody, ccnd1a antibody, bcl1 antibody, d11s287e antibody, u21b31 antibody, CCND1 antibody, cyclin D1 antibody, cyclin D1 S homeolog antibody, cyclin D1 L homeolog antibody, CCND1 antibody, Ccnd1 antibody, ccnd1 antibody, ccnd1.S antibody, ccnd1.L antibody

背景

Synonyms: cD1, CycD1, Cyl-1, PRAD1, bcl-1, AI32739, G1/S-specific cyclin-D1, Ccnd1
Background: Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).

基因ID

12443

UniProt

P25322

途径

PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling