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PD-L1 抗体 (AA 19-238)

Name: PD-L1 抗体 (AA 19-238)
Brand: antibodies-online
SKU: ABIN7164674
Availability: OutOfStock

This anti-PD-L1 antibody is a 小鼠单克隆 antibody detecting PD-L1 in WB, FACS, IHC, ELISA 和 IF. Suitable for 人.

Western Blot Positive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate All lanes: PD-L1 antibody at 1:2500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 55 kDa (PD-L1 抗体 (AA 19-238))

Overlay histogram showing A549 cells stained with ABIN7164674 (red line) at 1:150. (PD-L1 抗体 (AA 19-238))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate All lanes: PD-L1 antibody at 1:1000, 1:2000, 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 33 kDa Observed band size: 55 kDa (PD-L1 抗体 (AA 19-238))

IHC image of ABIN7164674 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (PD-L1 抗体 (AA 19-238))

IHC

IHC image of ABIN7164674 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. (PD-L1 抗体 (AA 19-238))

IHC

Immunofluorescence staining of 293 cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 抗体 (AA 19-238))

Immunofluorescence staining of A549 cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 抗体 (AA 19-238))

Immunofluorescence staining of Hela cells with ABIN7164674 at 1:100, counter-stained with DAPI. (PD-L1 抗体 (AA 19-238))

Overlay histogram showing 293 cells stained with ABIN7164674 (red line) at 1:150. (PD-L1 抗体 (AA 19-238))

FACS

9 images

产品编号 ABIN7164674

发货至: 中国

Quick Overview for PD-L1 抗体 (AA 19-238) (ABIN7164674)

抗原

See all PD-L1 抗体

PD-L1 (CD274 (PD-L1))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This PD-L1 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

克隆位点

9B5F4

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AA 19-238

交叉反应

人

纯化方法

Protein G purified

免疫原

Recombinant Human Programmed cell death 1 ligand 1 protein (19-238AA)

亚型

IgG2b
anti-CD274 (PD-L1) (C-Term) antibody
Verified PD-L1 适用: 人 WB, FACS, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 39-191) antibody
PD-L1 适用: 人, 小鼠 WB, FACS, IHC, ELISA, IF, Coat, StM 宿主: 小鼠 Monoclonal PDL1-2746 unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 适用: 人 FACS, IHC, ELISA 宿主: 小鼠 Monoclonal 6D9A2 unconjugated

anti-CD274 (PD-L1) (AA 48-290) antibody
PD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 34-241) antibody
PD-L1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 19-238) antibody
PD-L1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (C-Term) antibody
KO Validated PD-L1 适用: 人 WB, FACS, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-153) antibody
PD-L1 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 7D2A10 unconjugated

anti-CD274 (PD-L1) (AA 201-290) antibody
PD-L1 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-CD274 (PD-L1) (AA 24-234) antibody
PD-L1 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:100-1:300,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

PD-L1 (CD274 (PD-L1))

别名

PD-L1

背景

Background: Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/CD279, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443). The PDCD1/CD279-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077)
Aliases: Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274), CD274, B7H1 PDCD1L1 PDCD1LG1 PDL1

UniProt

Q9NZQ7

途径

Cancer Immune Checkpoints