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Hsc70 抗体 (AA 2-646)

Name: Hsc70 抗体 (AA 2-646)
Brand: antibodies-online
SKU: ABIN7154883
Availability: OutOfStock

This anti-Hsc70 antibody is a 小鼠单克隆 antibody detecting Hsc70 in WB, IHC, ELISA, IP 和 FACS. Suitable for 人.

Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s (Hsc70 抗体 (AA 2-646))

Overlay histogram showing MCF-7 cells stained with ABIN7154883 (red line). (Hsc70 抗体 (AA 2-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (Hsc70 抗体 (AA 2-646))

Western Blot Positive WB detected in: 20 μg Hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s (Hsc70 抗体 (AA 2-646))

IHC image of ABIN7154883 diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (Hsc70 抗体 (AA 2-646))

IHC

Immunoprecipitating HSPA8 in Hela whole cell lysate Lane 1: Mouse control IgG2b instead of ABIN7154883 in Hela whole cell lysate. (Hsc70 抗体 (AA 2-646))

7 images

产品编号 ABIN7154883

发货至: 中国

Quick Overview for Hsc70 抗体 (AA 2-646) (ABIN7154883)

抗原

See all Hsc70 (HSPA8) 抗体

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

适用

人

宿主

小鼠

克隆类型

单克隆

标记

This Hsc70 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

克隆位点

2G8F6

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AA 2-646

交叉反应

人, 小鼠, 大鼠

纯化方法

Protein A purified

免疫原

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

亚型

IgG2b
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 13-31), (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, IHC, ICC, IF, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (Internal Region) antibody
Verified HSPA8 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody (HRP)
HSPA8 适用: 人 WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 HRP

应用备注

Recommended dilution: WB: 1:10000-1:256000, IHC: 1:100-1:500, FC: 1:100-1:300, IP: 1 μL-4 μL,

限制

仅限研究用
状态

Liquid

缓冲液

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
抗原

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

别名

HSPA8

背景

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10

UniProt

P11142