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Hsc70 抗体 (N-Term)

The 兔多克隆 anti-Hsc70 antibody is suitable to detect Hsc70 in samples from 人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼和山羊. It has been validated for WB 和 IHC.

Sample type: rat brain lysatePrimary Dilution: 1:1000Image Submitted By: Jennifer Morgan, PhD &, David BuschUniversity of Texas in Austin (Hsc70 抗体 (N-Term))

Sample Type: Mouse Brain Slices Red: primaryBlue: DAPIPrimary Dilution: 1:400 Secondary Antibody: Anti-Rabbit IgG Alexa 594Secondary Dilution: 1:400 Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt University of Seville (Hsc70 抗体 (N-Term))

IHC

Host: Rabbit Target Name: HSPA8 Sample Tissue: Human 293T cell Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5. (Hsc70 抗体 (N-Term))

Host: Rabbit Target Name: HSPA8 Sample Tissue: Human Fetal Liver Antibody Dilution: 1. (Hsc70 抗体 (N-Term))

Host: Rabbit Target Name: RHOT1 Sample Type: Jurkat Antibody Dilution: 1. (Hsc70 抗体 (N-Term))

Host: Rabbit Target Name: NOP56 Sample Type: MCF7 Antibody Dilution: 1. (Hsc70 抗体 (N-Term))

IHC

Host: Rabbit Target Name: HIRIP3 Sample Type: 293T Antibody Dilution: 1. (Hsc70 抗体 (N-Term))

Rabbit Anti-HSPA8 Antibody Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue Observed Staining: Nuclear in pinealocytes Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0. (Hsc70 抗体 (N-Term))

IHC

10 images

产品编号 ABIN2783374

发货至: 中国

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中国
北京 101111

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Quick Overview for Hsc70 抗体 (N-Term) (ABIN2783374)

抗原

See all Hsc70 (HSPA8) 抗体

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

适用

人, 大鼠, 小鼠, Cow, 绵羊, Pig, 斑马鱼, 山羊

宿主

兔

克隆类型

多克隆

标记

This Hsc70 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product HSPA8 Primary Antibody

抗原表位
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N-Term

原理

HSPA8 Antibody - N-terminal region

序列

MSKGPAVGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL

预测反应

Cow: 100%, Goat: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

产品特性

This is a rabbit polyclonal antibody against HSPA8. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human HSPA8
anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 1-300) antibody
HSPA8 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 小鼠 Monoclonal 6E4D2 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 2-646) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IP, FACS 宿主: 小鼠 Monoclonal 2G8F6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (C-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 539-569), (C-Term) antibody
HSPA8 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16528 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 82-110), (N-Term) antibody
HSPA8 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB17655 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 适用: 人 WB, IHC, ELISA, IF, ICC, IP, Bind, PLA, AA 宿主: 小鼠 Monoclonal 1F2-H5 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 520-614) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS 宿主: 小鼠 Monoclonal 3B6 unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 适用: 人, 大鼠, 小鼠 WB, IHC, IF, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (Internal Region) antibody
Verified HSPA8 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 山羊 Polyclonal unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

说明

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48445-100UG

限制

仅限研究用
状态

Liquid

浓度

0.5 mg/mL

缓冲液

Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

prevent freeze-thaw cycles

储存条件

4 °C,-20 °C

储存方法

For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.
抗原

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

别名

HSPA8

背景

Background Information: HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. The protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.
Gene Name: Heat shock 70 kDa protein 8
Alternative Symbols: LAP1, HSC54, HSC70, HSC71, HSP71, HSP73, LAP-1, NIP71, HEL-33, HSPA10, HEL-S-72p
Protein Name: Heat shock cognate 71 kDa protein

分子量

71kDa

基因ID

3312

NCBI登录号

NP_006588

UniProt

P11142