RASGRF1 抗体 (pSer916)
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北京 101111
Quick Overview for RASGRF1 抗体 (pSer916) (ABIN7138730)
抗原
See all RASGRF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer916
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原理
- Phospho-RASGRF1 (Ser916) Antibody
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交叉反应
- 小鼠, 大鼠
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纯化方法
- Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
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免疫原
- Peptide sequence around phosphorylation site of Serine 916(N-E-G(p)-T P) derived from Human Ras-GRF1.
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亚型
- IgG
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anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (Middle Region) antibody
RASGRF1 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 1-190) antibodyRASGRF1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibodyRASGRF1 适用: 人 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 107-156) antibodyRASGRF1 适用: 小鼠, 人, Cow, 犬, 豚鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 467-516) antibodyRASGRF1 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, 猴, Pig, Bat, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibodyRASGRF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 907-936) antibodyRASGRF1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB14017 unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibodyRASGRF1 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))
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别名
- RASGRF1
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背景
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Background:
Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx . Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity. Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity. Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity.
Huibin Yang, J. Biol. Chem., Apr 2003, 278: 13278 - 13285.
Huibin Yang, Mol. Biol. Cell, May 2006, 17: 2177 - 2189.
Raymond R. Mattingly, J. Biol. Chem., Dec 1999, 274: 37379.
Tie-Nian Zhu, J. Biol. Chem., May 2007, 282: 14816 - 14826.Aliases: CDC25 antibody, CDC25L antibody, GNRP antibody, GRF1 antibody, GRF55 antibody, Guanine nucleotide exchange factor antibody, Guanine nucleotide releasing factor 55 kD antibody, Guanine nucleotide releasing protein antibody, guanine nucleotide-releasing factor 1 antibody, Guanine nucleotide-releasing protein antibody, H GRF55 antibody, HGRF55 antibody, PP13187 antibody, Ras protein specific guanine nucleotide releasing factor 1 antibody, Ras specific guanine nucleotide releasing factor antibody, Ras specific guanine nucleotide releasing factor CDC25 homolog antibody, Ras specific nucleotide exchange factor CDC25 antibody, Ras-GRF1 antibody, Ras-specific guanine nucleotide-releasing factor 1 antibody, Ras-specific nucleotide exchange factor CDC25 antibody, RASGRF1 antibody, RGRF1_HUMAN antibody
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UniProt
- Q13972
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途径
- RTK signaling, Cell Division Cycle, M Phase
抗原
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