Recombinant POLR2A/RPB1 抗体 (pSer5)
Quick Overview for Recombinant POLR2A/RPB1 抗体 (pSer5) (ABIN7127726)
抗原
See all POLR2A/RPB1 (POLR2A) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer5
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交叉反应
- 人
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纯化方法
- Affinity-chromatography
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免疫原
- A synthesized peptide derived from human Phospho-POLR2A (S5)
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亚型
- IgG
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anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibody
POLR2A 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 1-260) antibodyPOLR2A 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Polyclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer5) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IF, ELISA, ChIP, FM 宿主: 小鼠 Monoclonal unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (pSer2) antibodyPOLR2A 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 兔 Monoclonal 2G1 unconjugated Recombinant Antibody
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 128-258) antibodyPOLR2A 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 1B1D8 unconjugated
anti-RNA Polymerase II Subunit RPB1 (POLR2A) (AA 76-298) antibodyPOLR2A 适用: 人 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200,
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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- POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))
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别名
- POLR2A
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背景
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Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity).
Aliases: DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2A, POLR2
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UniProt
- P24928
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途径
- Regulatory RNA Pathways
抗原
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