Recombinant GLG1 抗体
Quick Overview for Recombinant GLG1 抗体 (ABIN7127258)
抗原
See all GLG1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.
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交叉反应 (详细)
- Human. Does not react with Mouse or Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human GLG1 protein
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亚型
- IgG
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- GLG1 (Golgi Glycoprotein 1 (GLG1))
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别名
- GLG1
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背景
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CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand-1 (ESL-1), Golgi Glycoprotein 1 (GLG1), Golgi apparatus protein 1, Golgi sialoglycoprotein MG-160, Slectin, endothelial cell, ligand,Golgi Complex (Marker for Human Cells)
Cellular localisation: Golgi complex in cytoplasm -
分子量
- 134kDa
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基因ID
- 2734, 109731
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UniProt
- Q92896
抗原
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