RBM8A 抗体
Quick Overview for RBM8A 抗体 (ABIN7127238)
抗原
See all RBM8A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Y14 Antibody is a high quality monoclonal Y14 antibody (also designated Y14 antibody) suitable for the detection of the Y14 protein of mouse, rat, human and Xenopus laevis origin. Y14 Antibody (4C4) is available as both the non-conjugated anti-Y14 antibody form, as well as multiple conjugated forms of anti-Y14 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor conjugates. The exon junction complex (EJC) is a multiprotein complex that assembles approximately 20-24 nucleotides upstream of exon-exon junctions in pre-mRNAs. It is involved in mRNA export, cytoplasmic localization, and nonsense-mediated mRNA decay. Members of the EJC include Y14, Aly/REF, Magoh, RNPS1, SRm160, and DEK. Aly/REF, Magoh, and Y14, identified as RBM8 in mouse and rat, make up the core of the EJC, and these proteins remain stably bound to spliced mRNAs in the cytoplasm until they are translated. Therefore, Y14, Aly/REF, and Magoh have the ability to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. The gene encoding human Y14 encodes three transcripts. Y14 is a ubiquitously expressed protein. Although Y14 shuttles to the cytoplasm, it is predominantly detected in the nucleus and is co-localized with oskar mRNA at the posterior pole of the cell.
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交叉反应 (详细)
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human RBM8A protein
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亚型
- IgG1
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or HepG2 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- RBM8A (RNA Binding Motif Protein 8A (RBM8A))
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别名
- RBM8A
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背景
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Binder of OVCA1-1, BOV1A, BOV1B, BOV1C, HSPC114, MDS014, ZRNP1, Ribonucleoprotein RBM8A, RNA-binding protein 8A, RNA-binding protein Y14,Y14 / RBM8A
Cellular localisation: Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. -
分子量
- 24kDa
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基因ID
- 9939, 728824
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UniProt
- Q9Y5S9
抗原
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