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CD63 抗体

CD63 适用: 人 FACS, IF, ELISA, IHC (f), IP, Func 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN7127224
发货至: 中国
  • 抗原 See all CD63 抗体
    CD63
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    小鼠
    克隆类型
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    单克隆
    标记
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    This CD63 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Functional Studies (Func)
    特异性
    CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
    交叉反应 (详细)
    Human. Porcine.
    纯化方法
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    免疫原
    Full-length human CD63 protein
    亚型
    IgG1
    Top Product
    Discover our top product CD63 Primary Antibody
  • 应用备注

    Known_Application: ELISA; Functional studies; Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)); ,Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

    Positive_Control: HL60, THP-1 or NIH/3T3 cells. Human spleen, melanoma or lymphoma.

    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    储存液
    Azide free
    储存条件
    -20 °C,-80 °C
    储存方法
    Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    有效期
    24 months
  • 抗原
    CD63
    别名
    CD63 (CD63 产品)
    别名
    C75951 antibody, ME491 antibody, Tspan30 antibody, LAMP-3 antibody, MLA1 antibody, OMA81H antibody, TSPAN30 antibody, TSP29FA antibody, CD63 antigen antibody, CD63 molecule antibody, Cd63 molecule antibody, Cd63 antibody, CD63 antibody
    背景
    Gp55, granulophysin, Lysosomal-associated membrane protein 3, Mast cell antigen AD1Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, NGA, Ocular melanoma-associated antigen, Tetraspanin-30, TSPAN30,CD63 (Late Endosomes Marker)
    Cellular localisation: Cell surface. Cytoplasm.
    分子量
    26kDa (core protein), 30-60kDa (glycosylated)
    基因ID
    967, 445570
    UniProt
    P08962
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