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CD63 抗体

CD63 适用: 人, 小鼠 FACS, WB, IHC, IF, ISt, StM 宿主: 小鼠 Monoclonal MX-49-129-5 unconjugated
产品编号 ABIN6941230
发货至: 中国
  • 抗原 See all CD63 抗体
    CD63
    适用
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    人, 小鼠
    宿主
    • 75
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    小鼠
    克隆类型
    • 96
    • 47
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    单克隆
    标记
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    This CD63 antibody is un-conjugated
    应用范围
    • 90
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    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt), Staining Methods (StM)
    特异性
    This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
    纯化方法
    Purified by Protein A/G
    免疫原
    Full length CD63 of human origin
    克隆位点
    MX-49-129-5
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD63 Primary Antibody
  • 应用备注

    Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    CD63
    别名
    CD63 (CD63 产品)
    别名
    C75951 antibody, ME491 antibody, Tspan30 antibody, LAMP-3 antibody, MLA1 antibody, OMA81H antibody, TSPAN30 antibody, TSP29FA antibody, CD63 antigen antibody, CD63 molecule antibody, Cd63 molecule antibody, Cd63 antibody, CD63 antibody
    分子量
    26kDa (core protein), 30-60kDa (glycosylated)
    基因ID
    967
    UniProt
    P08962
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