Recombinant CD163 抗体 (AA 1056-1156)
Quick Overview for Recombinant CD163 抗体 (AA 1056-1156) (ABIN7127171)
抗原
See all CD163 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1056-1156
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特异性
- CD163 is a type I membrane protein, and is a member of the hemoglobin scavenger receptor cystein-rich superfamily. The protein is involved in the clearance of hemoglobin-haptoglobin complexes and is considered to have anti-inflammatory functions. CD163 expression is restricted to the monocytic/macrophage lineage. It is expressed by all circulating monocytes and by a majority of tissue macrophages, such as splenic dendrocytes, alveolar macrophages and Kupffer cells of the liver. It is not present in macrophages in the mantle zone and some of the germinal center cells in lymph follicles, nor in Langerhans cells and interdigitating reticulum cells. In tumor tissues, CD163 is found in almost all cases of acute myeloid leukemia with monocytoid differentiation and in the majority of cases of histiocytic sarcoma, littoral cell angioma, Rosai-Dorfman disease, Langerhans cell histiocytosis and typical and atypical fibrous histiocytoma. It is also expressed in some cases of dermatofibrosarcoma protuberans. CD163 can be used to detect cells of monocytic and histiocyte lineage in neoplastic and reactive lesions. It has been shown to be more sensitive than CD68 for the detection of macrophages and monocytic cells. It covers a similar, but not identical, spectrum of cells as CD68.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Synthetic peptide corresponding to CD163 residues within aa1056-1156 of CD163 was used as an immunogen.
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亚型
- IgG
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anti-CD163 (CD163) (AA 1001-1121) antibody
CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1001-1121) antibody (FITC)CD163 适用: 人, 小鼠, 大鼠, Pig, 犬 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal FITC
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 IHC, ELISA, ISt, Coat, StM 宿主: 小鼠 Monoclonal M130-2162 unconjugated
anti-CD163 (CD163) (AA 43-196) antibodyCD163 适用: 人 WB, IHC, ISt, StM 宿主: 小鼠 Monoclonal M130-1210 unconjugated
anti-CD163 (CD163) (AA 42-259) antibodyCD163 适用: 人, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 7G5E2 unconjugated
anti-CD163 (CD163) (AA 697-972) antibodyCD163 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) antibodyCD163 适用: 人 WB, FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal GHI-61 unconjugated
anti-CD163 (CD163) (AA 47-201) antibodyCD163 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 1092-1109), (C-Term) antibodyCD163 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-CD163 (CD163) (AA 693-939) antibodyCD163 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: hPBL cells. Skin, lung, placenta or histiocytoma tissue.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD163
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别名
- CD163
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背景
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Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130,CD163 (Monocyte & Macrophage Marker)
Cellular localisation: Cell Surface & Cytoplasmic -
分子量
- 140kDa
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基因ID
- 9332, 504641
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UniProt
- Q86VB7
抗原
-
