CD20 抗体 (AA 266-297)
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Quick Overview for CD20 抗体 (AA 266-297) (ABIN7127162)
抗原
See all CD20 (MS4A1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 266-297
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特异性
- Recognizes a protein of 30-33 kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.
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交叉反应 （详细）
- Human, Mouse and Rat.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A synthetic peptide from the C-terminal region of human CD20 protein (around aa 266-297) (exact sequence is proprietary)
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亚型
- IgG1
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anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (C-Term) antibody
Verified MS4A1 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 213-297) antibodyMS4A1 适用: 人 FACS, WB, IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MS4A1-3409 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal L26 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal L26-IGEL-773 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 FACS, WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal rIGEL-773 unconjugated Recombinant Antibody
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibodyMS4A1 适用: 人, Cow FACS, IP 宿主: 小鼠 Monoclonal MEM-97 unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 1-297) antibodyMS4A1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) (AA 210-297) antibodyMS4A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5C11 unconjugated
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应用备注
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Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Daudi, Raji or U266 cells. Human lymphocytes. Human lymph node or tonsil.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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别名
- MS4A1
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背景
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APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, MS4A1, MS4A2,CD20 / MS4A1 (B-Cell Marker)
Cellular localisation: Cell Surface -
分子量
- 33-37kDa
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基因ID
- 931, 712553
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UniProt
- P11836
抗原
-
