Recombinant Histone H3.3 抗体
Quick Overview for Recombinant Histone H3.3 抗体 (ABIN7127092)
抗原
See all Histone H3.3 (H3F3A) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10)
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亚型
- IgG1
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anti-H3 Histone, Family 3A (H3F3A) (AA 2-136) antibody
H3F3A 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (AA 1-137) antibodyH3F3A 适用: 人 ELISA, WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 2D7-H1 unconjugated
anti-H3 Histone, Family 3A (H3F3A) (Lys9) antibodyH3F3A 适用: 人 ELISA, WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (pThr3) antibodyH3F3A 适用: 人 ELISA, WB, ICC, FACS 宿主: 兔 Monoclonal 28H4 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 ELISA, IHC, IF 宿主: 兔 Monoclonal 4H2 unconjugated Recombinant Antibody
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) antibodyH3F3A 适用: 人 WB, IHC (p) 宿主: 兔 Monoclonal 7B12 unconjugated
anti-H3 Histone, Family 3A (H3F3A) (2meLys79) antibodyH3F3A 适用: 人 ELISA, WB, ChIP 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) (AA 100-150) antibodyH3F3A 适用: 人, 小鼠, Cow, Drosophila melanogaster, 马, 小鸡, Opossum WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-H3 Histone, Family 3A (H3F3A) antibodyMicroarray verified H3F3A 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB199456 unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human tonsil, liver or breast carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))
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别名
- H3F3
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背景
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Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, Histone cluster 1, H3a, Histone H3 3 pseudogene, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l,Histone H3, phosphorylated (pSer10) (Nuclear Marker)
Cellular localisation: Nucleus. Chromosome. -
分子量
- 15kDa
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基因ID
- 8350, 132854
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UniProt
- P68431
抗原
-
