SRF 抗体
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北京 101111
Quick Overview for SRF 抗体 (ABIN7126982)
抗原
See all SRF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human SRF protein
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亚型
- IgG1
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anti-Serum Response Factor (SRF) (AA 101-250) antibody
SRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (pSer99) antibodySRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 406-508) antibodySRF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1E1 unconjugated
anti-Serum Response Factor (SRF) (N-Term) antibodySRF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 406-508) antibodySRF 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Serum Response Factor (SRF) (AA 57-269) antibodySRF 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (pSer77) antibodySRF 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (AA 231-474) antibodySRF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Serum Response Factor (SRF) (N-Term) antibodySRF 适用: 人, 小鼠, 大鼠, Pig, 犬, Cow, 兔, 斑马鱼 WB, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, U87 or Jurkat cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- SRF (Serum Response Factor (SRF))
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别名
- SRF
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背景
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C fos serum response element binding transcription factor, ELK3, ERP, MCM1, SAP2, Serum response factor (SRF), SRF serum response factor c fos serum response element binding transcription factor,Serum Response Element Binding Transcription Factor (SRF)
Cellular localisation: Nucleus. Nucleoplasm. -
分子量
- 40-67kDa
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基因ID
- 6722, 520140
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UniProt
- P11831
抗原
-
