SRF 抗体
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- 抗原 See all SRF 抗体
- SRF (Serum Response Factor (SRF))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This SRF antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Coating (Coat), Flow Cytometry (FACS)
- 特异性
- Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1), have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino-terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.
- 交叉反应 (详细)
- Human.
- 纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- 免疫原
- Recombinant full-length human SRF protein
- 亚型
- IgG1
- Top Product
- Discover our top product SRF Primary Antibody
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- 应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, U87 or Jurkat cells.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- 储存液
- Azide free
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- 有效期
- 24 months
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- 抗原
- SRF (Serum Response Factor (SRF))
- 别名
- SRF (SRF 产品)
- 背景
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C fos serum response element binding transcription factor, ELK3, ERP, MCM1, SAP2, Serum response factor (SRF), SRF serum response factor c fos serum response element binding transcription factor,Serum Response Element Binding Transcription Factor (SRF)
Cellular localisation: Nucleus. Nucleoplasm. - 分子量
- 40-67kDa
- 基因ID
- 6722, 520140
- UniProt
- P11831
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