Recombinant Smooth Muscle Actin 抗体
Quick Overview for Recombinant Smooth Muscle Actin 抗体 (ABIN7126869)
抗原
See all Smooth Muscle Actin (ACTA2) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
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特异性
- Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human ACTA2 protein
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亚型
- IgG
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anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody
Verified ACTA2 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyACTA2 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, ELISA, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyKO Validated ACTA2 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyACTA2 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 1A4-ACTA2-791 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-124) antibodyACTA2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 2-124) antibodyACTA2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5C1H11 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibodyACTA2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 大鼠, 小鼠, 小鸡, 兔, 山羊 WB, IHC, ELISA, IHC (p), ICC, IHC (fro), IEM 宿主: 小鼠 Monoclonal alpha-SM1-1A4 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-377) antibodyACTA2 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4A8-2H3 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 猴 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4F4 unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
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别名
- ACTA2
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背景
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ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha Cardiac Actin, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46,Actin, Smooth Muscle (Leiomyosarcoma Marker)
Cellular localisation: Cytoplasmic -
分子量
- 42kDa
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基因ID
- 59, 500483
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UniProt
- P62736
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途径
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
抗原
-
