OCT4 抗体 (AA 1-134)
Quick Overview for OCT4 抗体 (AA 1-134) (ABIN7126815)
抗原
See all OCT4 (POU5F1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-134
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特异性
- Oct4 Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
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交叉反应 （详细）
- Human
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant fragment of human OCT-4/POU5F1 protein (around aa 1-134) (exact sequence is proprietary)
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亚型
- IgG2b
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anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibody
POU5F1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyKO Validated POU5F1 适用: 人 WB, IF, ICC, IP, FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 201-300) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 小鼠 Monoclonal 2H41D8 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 7E7 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 136-360) antibodyPOU5F1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 1-360) antibodyPOU5F1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (AA 81-164) antibodyPOU5F1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D2 unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) (Internal Region) antibodyPOU5F1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-POU Class 5 Homeobox 1 (POU5F1) antibodyPOU5F1 适用: 人, 猴 WB, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Raji or U-937 cells. Human seminoma or testicular carcinoma
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))
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别名
- POU5F1
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背景
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OCT3, OCT3A, OCT4, OCT4A, OCT-3/4, Octamer-binding protein 3, Octamer-binding protein 4, Octamer-binding transcription factor 3, octamer-binding transcription factor-3, OTF-3, OTF3, OTF4, PO5F1, POU class 5 homeobox 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, POU5F1,OCT-4 (POU5F1) (Cancer Stem Cell Marker)
Cellular localisation: Nucleus -
分子量
- 38.6kDa
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基因ID
- 5460, 249184
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UniProt
- Q01860
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途径
- Stem Cell Maintenance
抗原
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