POLE3 抗体
Quick Overview for POLE3 抗体 (ABIN7126801)
抗原
See all POLE3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.
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交叉反应 （详细）
- Human. Predicted to react in Mouse, Rat and Xenopus.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human POLE3 protein
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亚型
- IgG1
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anti-Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (AA 1-147) antibody
POLE3 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (N-Term) antibodyPOLE3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 斑马鱼, 马, 兔, 猴, Hamster, Pig, Bat, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (C-Term) antibodyPOLE3 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 犬, 猴, Hamster, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) antibodyPOLE3 适用: 人, 小鼠, 大鼠 WB, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) (AA 5-34), (N-Term) antibodyPOLE3 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB18580 unconjugated
anti-Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) antibodyPOLE3 适用: 人, 小鼠, 猴 WB, IF, IP, ICC 宿主: 小鼠 Monoclonal 3C5-1 unconjugated
anti-Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3) antibodyPOLE3 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa or K562 cells.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))
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别名
- POLE3
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背景
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Arsenic transactivated protein, ASTP, CHRAC17, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, Histone fold protein CHRAC17, HuCHRAC17, Polymerase (DNA directed) epsilon 3 (POLE3), YBL1,POLE3 / CHRAC17
Cellular localisation: Nucleus. Cytoplasm. -
分子量
- 16.86kDa
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基因ID
- 54107, 108112
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UniProt
- Q9NRF9
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途径
- DNA Damage Repair
抗原
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