TDP2 抗体
Quick Overview for TDP2 抗体 (ABIN7126778)
抗原
See all TDP2 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This MAb recognizes a protein of 41kDa, which is identified as TDP2. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human TDP2 protein
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亚型
- IgG2b
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anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 1-362) antibody
TDP2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3D11 unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 1-362) antibodyTDP2 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 246-272), (C-Term) antibodyTDP2 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB20005 unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (Middle Region) antibodyTDP2 适用: 人, 小鼠, 大鼠, 兔, Cow, 犬, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 253-281), (C-Term) antibodyTDP2 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) antibodyTDP2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 154-203) antibodyTDP2 适用: 人, 小鼠, 大鼠, 兔, 豚鼠, Bat, 猴, 斑马鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) antibodyGMP Grade TDP2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 42c unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (AA 29-305) antibodyTDP2 适用: 人 WB, IHC, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) antibodyTDP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Lung, Duodenum or Small Intestinal Carcinoma.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))
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别名
- TDP2
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背景
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5'-Tyr-DNA phosphodiesterase, EAP2, EAPII, ETS1-associated protein 2, ETS1-associated protein II, TRAF and TNF receptor-associated protein, TTRAP, Tyr-DNA phosphodiesterase 2, Tyrosyl-DNA phosphodiesterase 2 (TDP2),TDP2 / EAPII
Cellular localisation: Nuclear and Cytoplasmic -
分子量
- 41kDa
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基因ID
- 51567, 728795
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UniProt
- O95551
抗原
-
