NME1 抗体
Quick Overview for NME1 抗体 (ABIN7126749)
抗原
See all NME1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.
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交叉反应 （详细）
- Human.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length human NME1 protein
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亚型
- IgG2b
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anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 43-152) antibody
NME1 适用: 人 WB, ELISA, IHC (p), IF, IP 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 1-152) antibodyNME1 适用: 人 WB, ELISA, IF, RNAi 宿主: 小鼠 Monoclonal 2H1 unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) antibodyNME1 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4H2 unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) antibodyNME1 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 4B2 unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (N-Term) antibodyNME1 适用: 人, 小鼠, 大鼠, Cow, 马, 兔, 豚鼠 WB, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 6-147) antibodyNME1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (N-Term) antibodyNME1 适用: 人, 小鼠, 大鼠, 犬, 猴, Pig, Cow, 马, 兔, 小鸡, 非洲爪蟾, 斑马鱼, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (C-Term) antibodyNME1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (N-Term) antibodyNME1 适用: 人, 小鼠, 大鼠, 犬, Pig, Cow, 小鸡, 非洲爪蟾, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1) (AA 137-152), (C-Term) antibodyNME1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: ELISA (For coating, order antibody without BSA), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, A549 or Jurkat cells. Ubiquitously expressed in all tissues.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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储存液
- Azide free
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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有效期
- 24 months
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- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
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别名
- NME1
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背景
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AWD, Granzyme A activated DNase (GAAD), GZMA activated DNase, Metastasis inhibition factor NM23, NBS, NDKA, NM23, nm23-H1, NM23-M1, NM23H1B, included, NME/NM23 nucleoside diphosphate kinase 1, Nme1, NME1-NME2 spliced read-through transcript, included, Non-metastatic cells 1, protein (NM23A) expressed in, Nonmetastatic cells 1, protein expressed in, Nonmetastatic protein 23, Tumor metastatic process-associated protein,Nucleoside Diphosphate Kinase A / nm23-H1
Cellular localisation: Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization. -
分子量
- 17kDa
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基因ID
- 4830, 463456
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UniProt
- P15531
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途径
- Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
抗原
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